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首款專注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array

【字體：大中小】 時(shí)間：2016年12月08日 來源：康成生物

編輯推薦：

　　Arraystar最新發(fā)布了市場(chǎng)上首款專注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array。該款芯片可同時(shí)檢測(cè)66個(gè)細(xì)胞核tRNA和22個(gè)線粒體tRNA，覆蓋了tRNA權(quán)威數(shù)據(jù)庫GtRNAdb和tRNAdb中的所有反密碼子，方便客戶快速地進(jìn)行tRNA表達(dá)譜分析。

立即索取nrStar Human tRNA Repertoire PCR Array的詳細(xì)資料

轉(zhuǎn)運(yùn)RNA (tRNA) 是生物體內(nèi)分布最廣泛、含量最豐富的非編碼小RNA分子。它攜帶并轉(zhuǎn)運(yùn)氨基酸，參與蛋白翻譯，是連接mRNA與蛋白質(zhì)的重要橋梁。細(xì)胞增殖[1]、分化[1, 2]和凋亡[3]等一系列生物學(xué)過程都伴隨著tRNA水平的變化。反之，tRNA表達(dá)譜的改變也會(huì)影響細(xì)胞發(fā)育過程中的命運(yùn)抉擇。表達(dá)失調(diào)的tRNA可以促進(jìn)腫瘤的發(fā)生和癌癥進(jìn)程[2, 4-11]。另外，許多其它疾病比如II型糖尿病[12]、亨廷頓癥[13]以及HIV感染[14]都出現(xiàn)了tRNA表達(dá)與分布紊亂。tRNA研究已逐漸成為生物學(xué)過程和疾病研究的重要組成部分。

Arraystar最新發(fā)布了市場(chǎng)上首款專注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array。該款芯片可同時(shí)檢測(cè)66個(gè)細(xì)胞核tRNA和22個(gè)線粒體tRNA，覆蓋了tRNA權(quán)威數(shù)據(jù)庫GtRNAdb和tRNAdb中的所有反密碼子，方便客戶快速地進(jìn)行tRNA表達(dá)譜分析，為下一步的密碼子偏好性、蛋白翻譯效率和準(zhǔn)確性、細(xì)胞動(dòng)力學(xué)以及病毒感染的細(xì)胞嗜性等研究提供重要線索，是進(jìn)行tRNA研究必不可少的工具。

tRNA存在種類繁多的修飾，對(duì)其發(fā)揮功能十分關(guān)鍵。然而，這些修飾尤其是甲基化修飾會(huì)嚴(yán)重阻礙反轉(zhuǎn)錄的進(jìn)行，導(dǎo)致cDNA合成終止或堿基錯(cuò)配。為此，Arraystar專門開發(fā)了針對(duì)tRNA的反轉(zhuǎn)錄試劑盒(rtStar™ tRNA-optimized First-Strand Synthesis Kit)。該試劑盒采用了一種高效的RNA去甲基化酶AlkB，能夠有效的去除tRNA上的甲基化修飾，極大的提高cDNA合成質(zhì)量。與此試劑盒組合使用，研究人員能夠獲得更為真實(shí)可靠的tRNA表達(dá)變化，為研究蛋白質(zhì)組或tRNA來源片段（tRFs）提供重要信息。

為了保證數(shù)據(jù)可信度，芯片還包含了3個(gè)看家小RNA作為內(nèi)參，以及3個(gè)質(zhì)控對(duì)照RNA Spike-in、PCR陽性對(duì)照（PPC）和基因組DNA對(duì)照（GDC）,分別用于監(jiān)測(cè)cDNA合成質(zhì)量、PCR效率和基因組DNA污染。

在后續(xù)研究中，敲低tRNA[15] 或過表達(dá)tRNA [2, 16, 17]可以進(jìn)一步揭示特定tRNA在細(xì)胞生命活動(dòng)中的作用。其它用于非編碼RNA研究的相關(guān)技術(shù)也適用于tRNA的后續(xù)研究。

產(chǎn)品列表

產(chǎn)品名稱	規(guī)格	描述
nrStar™ Human tRNA Repertoire PCR Array	384-well (4*96) plate	包括66個(gè)細(xì)胞核tRNA和22個(gè)線粒體tRNA

芯片特點(diǎn)

• 囊括GtRNAdb和tRNAdb數(shù)據(jù)庫中所有的細(xì)胞核與線粒體反密碼子
• 伴隨的去甲基化處理使得檢測(cè)結(jié)果更加真實(shí)可靠
• 所有引物均在多種細(xì)胞和組織中通過驗(yàn)證
• 即拆即用型384孔板，幾小時(shí)內(nèi)便可得到結(jié)果

工作流程

適用實(shí)時(shí)定量熒光PCR儀：

ABI ViiA™ 7，ABI 7500 & ABI 7500 FAST，ABI 7900HT，ABI QuantStudio™ 6 Flex Real-Time PCR system，ABI QuantStudio™ 7 Flex Real-Time PCR system，ABI QuantStudio™ 12K Flex Real-Time PCR System，Bio-Rad CFX384，Bio-Rad iCycler & iQ Real-Time PCR Systems，Eppendorf Realplex，QIAGEN Rotor Gene Q100，Roche LightCycler 480，Stratagene Mx3000，Roche LightCycler 480

數(shù)據(jù)庫

tRNA反密碼子信息(GtRNAdb和tRNAdb數(shù)據(jù)庫)：

細(xì)胞核tRNA (66):

Ala-AGC, Ala-CGC, Ala-GGC, Ala-TGC, Arg-ACG, Arg-CCG, Arg-CCT, Arg-TCG, Arg-TCT, Asn-ATT, Asn-GTT, Asp-ATC, Asp-GTC, Cys-GCA, Gln-CTG-1, Gln-CTG-2, Gln-TTG-1, Gln-TTG-2, Glu-CTC, Glu-TTC, Gly-CCC-1, Gly-CCC-2, Gly-GCC, Gly-TCC, His-GTG, Ile-AAT, Ile-TAT, Ini-CAT, Leu-AAG, Leu-CAA, Leu-CAG, Leu-TAA, Leu-TAG, Lys-CTT-1, Lys-CTT-2, Lys-TTT, Met-CAT, Phe-GAA, Pro-AGG, Pro-CGG, Pro-GGG, Pro-TGG, Sec-TCA, Ser-ACT, Ser-AGA, Ser-CGA, Ser-GCT, Ser-GGA, Ser-TGA, Sup-CTA, Sup-TTA, Thr-AGT-1, Thr-AGT-2, Thr-CGT, Thr-TGT-1, Thr-TGT-2, Trp-CCA, Tyr-ATA, Tyr-GAT, Tyr-GTA, Val-AAC, Val-CAC-1, Val-CAC-2, Val-CAC-3, Val-TAC-1, Val-TAC-2

線粒體tRNA (22):

mt-Ala-TGC, mt-Arg-TCG, mt-Asn-GTT, mt-Asp-GTC, mt-Cys-GCA, mt-Gln-TTG, mt-Glu-TTC, mt-Gly-TCC, mt-His-GTG, mt-Ile-GAT, mt-Leu-TAA, mt-Leu-TAG, mt-Lys-TTT, mt-Met-CAT, mt-Phe-GAA, mt-Pro-TGG, mt-Ser-GCT, mt-Ser-TGA, mt-Thr-TGT, mt-Trp-TCA, mt-Tyr-GTA, mt-Val-TAC

立即索取nrStar Human tRNA Repertoire PCR Array的詳細(xì)資料

參考文獻(xiàn)
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